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GhKCBP immunoblotting in cotton fibers. Equal amounts of fiber total proteins were separated by SDS-PAGE and transferred to nitrocellulose. The upper part of the blot was probed with anti-GhKCBP antibodies, and the lower part was probed with an anti-α-tubulin antibody. Note that the GhKCBP level decreased from 10 to 21 DPA, whereas the tubulin level at least remained similar or showed some increase.
