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. 2003 May;132(1):174–184. doi: 10.1104/pp.102.016857

Figure 1.

Figure 1

Sequences of targeting oligonucleotides and strategy of gene correction by chimeric oligonucleotides. A, Oligonucleotide sequences. B, Schematic presentation of chimera-mediated gene conversion. The endogenous DNA repair system mediates conversion of G to T at position 1,719, gives rise to substitution of Trp to Leu, and leads to appearance of chlorsulfuron-resistant phenotype. Lowercase letters indicate 2′-O-methyl RNA residues, and uppercase letters indicate DNA bases. The mismatched base is underlined. The chimeric ChALS-588 and the DNA-composed oligonucleotide ChALS-587 are designed to make conversions at the codon for Pro-196, whereas chimera ChALS-1719 is designed to alter the codon for Trp-573. ChALS(−) is a nonspecific chimera.