Figure 3.

Confirmation of oligonucleotide-mediated acetolactate syntase gene conversion by sequence analysis. Nucleotide sequences from control wild type (WT) and independent resistant lines rchl-6.6, rchl-17, rchl-20, and rchl-3.11 are shown. G2 rchl-6.6 is sequence of haploid from line rchl-6.6. Plate A, Nucleotide substitutions at the codon for Trp-573. Plate B, Nucleotide substitutions at the codon for Pro-196. The targeted codon is underlined and conversion events are indicated with arrows.