Table II.
Assessment of wild-type and independent resistant lines after herbicide application and segregation of chlorsulfuron resistance in T1 progeny
| Line | Response on Application of 84 μm Chlorsulfuron
|
T1 Progeny Seedlings No. of Plants
|
||||
|---|---|---|---|---|---|---|
| Plant phenotype | Leaf color | Presence of necroses | Fresh weighta | Resistant | Sensitive | |
| g | ||||||
| WT (Pro-196 and Trp-573) | Sensitive | Bleached | +++ | 197.02 ± 8.36 a | 0 | 330 |
| rchl-3.11 (Leu-196) | Susceptible | Green | + | 214.6 ± 12.87 a | 149 | 61 |
| χ2 = 1.835 | ||||||
| rchl-20b (Thr-196) | Resistant | Green | – | 316.25 ± 9.27 c | 400 | 0 |
| rchl-17 (Ala-196) | Resistant | Green | – | 262.02 ± 5.31 b | 113 | 37 |
| χ2 = 0.009 | ||||||
| rchl-5.5 (Ser-196) | Resistant | Green | – | 254.12 ± 6.23 b | 167 | 53 |
| χ2 = 0.097 | ||||||
| rchl-6.6 (Leu-573) | Resistant | Green | – | 242.17 ± 15.92 b | 184 | 68 |
| χ2 = 0.529 | ||||||
| rchl-2.5 (Leu-573) | Resistant | Green | – | 237.02 ± 14.01 b | 175 | 65 |
| χ2 = 0.556 | ||||||
a
Values presented as a mean ± se from measurements of five independent plants. Within-column means without common letters are significantly different at P < 0.05.
b
Resistant dihaploid line. +, Indicates that some leaves had small necrotic spots; +++, indicates wide necroses up to dryness of the whole leaf blade. χ2 values were calculated on the basis of expected ratio of three resistant to one sensitive plant.