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A, Semiquantitative RT-PCR amplification of RNA extracted from cells during induction of the CCM. Amplification conditions and quantitation of band intensities were as described in “Materials and Methods.” No bands were detected in the minus RT controls (data not shown). B, Expression profiles from the RT-PCR reactions shown in A. The reference gene, ndhD1, was used to correct for errors in the estimation of RNA concentrations and for differences in RT reaction efficiency.
