Figure 1.

Time course of H₂O₂ effects under PhL on Ndh activity. A, Typical NADH-DH zymograms of the Ndh complex of crude extracts (60 μg of protein per lane) from 14-d-old barley leaf segments incubated up to 24 h under PhL (300 μmol photon m⁻² s⁻¹) and water or H₂O₂ (10 mm). B, Quantification of Ndh complex activity by image analysis of NADH-DH zymograms as shown in A. Activities were expressed as percentages of the value in freshly detached leaves (0 time). Each value represents the mean ± se of at least four independent experiments.