Figure 2.

Phosphorylation of the Ndh complex. Thylakoids were isolated from 14-d-old primary leaves incubated for 20 h under PhL (300 μmol photon m⁻² s⁻¹). After Triton X-100 solubilization, aliquots of 60 μg protein were subjected to native-PAGE, and NADH-DH zymograms were developed. After electroblotting onto polyvinylidene difluoride membranes, Ndh complex was detected with NDH-F antibody, and phosphorylated polypeptides were detected with a mixture of monoclonal phosphoamino acid (anti-phospho-Ser, anti-phospho-Thr, and anti-phospho-Tyr) antibodies.