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. 2006 Nov 7;34(21):6233–6246. doi: 10.1093/nar/gkl886

Figure 4.

Figure 4

Effect of double and triple dcl-mutations on DNA virus infection, siRNA biogenesis and VIGS. (A) Double mutants d2d3, d2d4, d3d4 and the triple mutant d2d3d4 1 month post-inoculation with CaLCuV::Chl. (B) Quantitative real-time RT–PCR for CaLCuV::Chl-infected double and triple mutants, performed as in Figure 1D. (C) LMW RNA blot analysis of Col-0 mock and CaLCuV::Chl-infected Col-0, dcl4, dcl-triple and double mutants. Two RNA sample pools are shown for infected d2d3d4, d2d3 and d2d4. (D) LMW RNA blot analysis of Col-0 mock and CaMV-infected Col-0, dcl4, d2d3d4 mutants. U6 signal is a loading control.