Table II.
Element contents of mesophyll protoplasts and vacuoplasts isolated from Cd- and SA-treated leaves
| Element Ratios | Mesophyll Protoplasts
|
Mesophyll Vacuoplasts
|
||
|---|---|---|---|---|
| Control | SA-Pretreated | Control | SA-Pretreated | |
| Cd/Ca (×10−4) | 17.3 ± 2.8 | 17.9 ± 0.8 | 55.1 ± 2.4 | 57.4 ± 2.8 |
| Cd/P (×10−4) | 8.7 ± 1.6 | 7.9 ± 1.2 | 6.1 ± 0.9 | 5.8 ± 1.0 |
Barley leaves were supplied with 500 μm SA or water for 24 h via the cut leaf sheath. Mesophyll protoplasts were isolated and suspended in sorbit medium supplemented with 25 μm CdCl2. Vacuoplasts were prepared after 4 h. Mesophyll protoplasts and mesophyll vacuoplasts were analyzed for element composition. The table provides the data on element ratios from three independent experiments ± sd.