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T.acidophilum MCM protein forms hexamers in solution. (A) An aliquot of 100 µg of purified T.acidophilum (taMCM) or M.thermautotrophicus (mtMCM) MCM protein was applied to a superose-6 gel-filtration column and analyzed as described in Materials and Methods. (B) The peak elution of the proteins in relation to the peak positions of thyroglobulin (Thy, 669 kDa), ferritin (Fer, 440 kDa), and gamma globulin (Gam, 158 kDa) is shown.
