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T.acidophilum Cdc6-2 protein stimulates MCM dsDNA translocation. Shown is a MCM helicase assay performed as described in Materials and Methods in the absence (A) or presence (B) of Cdc6-2 protein (2.7 pmol) and increasing amounts of MCM protein. Panels A and B, lane 1, boiled substrate; lane 2, substrate only; lane 3, 0.15 pmol of MCM; lane 4, 0.45 pmol of MCM; lane 5, 1.35 pmol of MCM; lane 6, 1.35 pmol of MCM K343A mutant as hexamer. Panel C, a summary of the data.
