Figure 1.

Nod factor treatment activates PA, PBut, and DGPP formation in suspension-cultured alfalfa cells. A, Nod factor stimulates the formation of PA and PBut in a dose-dependent manner. Cells were prelabeled with ³²P_i for 3 h and stimulated with different concentrations of Nod factor for 15 min in the presence of 0.5% (v/v) n-butanol. Treatment was stopped, and lipids were extracted, separated by TLC, and detected by autoradiography. B, Quantification of PA levels; C, quantification of PBut levels; and D, quantification of DGPP levels. The amount of radioactivity in each species was quantified by phosphor imaging and expressed as -fold stimulation compared with the control. Error bars indicate sds. E, Nod factor stimulates the formation of PA and PBut in a time-dependent manner. Cells were prelabeled with ³²P_i for 3 h and stimulated with 10⁻⁹ m Nod factor for different periods in the presence of 0.5% (v/v) n-butanol. Treatment was stopped, and lipids were extracted, separated by ethyl acetate TLC, and detected by autoradiography. F, Quantification of PA levels; G, quantification of PBut levels; and H, quantification of DGPP levels. The amount of radioactivity in each species was quantified by phosphor imaging and expressed as -fold stimulation compared with the control. Error bars indicate sds.