Figure 8.

RBPMS promotes the nuclear accumulation of Smad proteins. (A) Subcellular localization of RBPMS. Serum-starved 293T cells were treated with or without 5 ng/ml TGF-β1. The cells were then fractionated into cytoplasmic and nuclear fractions. The lysates were probed with the anti-RBPMS. Lamin A/C and α-tubulin were used as the nuclear and cytoplasmic marker, respectively. (B) RBPMS-induced nuclear accumulation of Smad proteins. Serum-starved 293T cells were transfected with 3 μg of the FLAG-tagged RBPMS expression vector or its empty vector, and treated with 5 ng/ml TGF-β1. The cells were fractionated as in (A). The lysates were detected with anti-FLAG, anti-Smad2/3 or anti-Smad4.