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. 2006 Nov 10;34(21):6256–6263. doi: 10.1093/nar/gkl755

Figure 4.

Figure 4

Increased Tra2 expression in females results in increased M1 retention. Low cycle RT–PCR analysis of M1 splicing in non-transgenic (nt) female adults and females carrying both hsp70-Gal4 and either of two UAS-MycTra2 insertions (lines 1 and 2) are shown. The percent of products with the M1 intron retained is indicated below the gel. The level of M1 retention is increased slightly without heat shock and more dramatically after heat shock and recovery. Reactions were performed both with (+) and without (−) reverse transcriptase. RNA samples are from the same individuals used for the western blot in Figure 3B.