Figure 5.

Oligomeric states of mitochondrial protein complexes as detected by immunoblotting. Proteins resolved by 2D-Tricine-SDS-PAGE gels were transferred onto nitrocellulose membranes and immunoblotted with the indicated antibodies (from top to bottom, anti-beta subunit of Polytomella sp. ATP synthase, anticore I of N. crassa, anti-COXIIB of Polytomella sp., and anti-AOX of C. reinhardtii). The arrow indicates the position of the well on the first dimension BN-PAGE, where a small portion of total proteins precipitates before entering the stacking gel.