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. 2003 May;132(1):372–380. doi: 10.1104/pp.102.019679

Figure 2.

Figure 2

Comparison of deduced amino acid sequences of methyltransferases. A, Alignment of coffee N-methyltransferases. Genes, substrates, and accession numbers are as follows: CaXMT1, xanthosine, AB048793; CaMXMT1, 7-methylxanthine, AB048794; CaMXMT2, 7-methylxanthine, AB084126; and CaDXMT1, theobromine, AB084125. The sequences were aligned by ClustalW (Thompson et al., 1994). Identical residues shared by at least three proteins are shaded in black, and conserved substitutes found in at least three proteins are shaded in gray. B, Unrooted phylogenetic tree of coffee N-methyltransferases with related proteins. Genes, substrates, species nomenclatures, and accession numbers are as follows: CaMTL1, unknown, coffee, AB039725; CaMTL2, unknown, coffee, AB048792; CTS1, 7-methylxanthine, coffee, AB034700; CTS2, 7-methylxanthine, coffee, AB054841; CCS1, theobromine, coffee, AB086414; TCS1, theobromine, tea, AB031280; JMT, jasmonic acid methyltransferase, Arabidopsis, AY008434; SAMT, salicylic acid carboxyl methyltransferase, Clarkia breweri, AF133053; BAMT, benzoic acid carboxyl methyltransferase, Antirrhinum majus, AF198492; PEAMT, phosphoethanolamine N-methyltransferase, Spinacia oleracea, AF237633; CNMT, coclaurine N-methyltransferase, Coptis japonica, AB061863; and PMT1, putrescine N-methyltransferase 1, Nicotiana tabacum, BAA05867. The sequences were compared using ClustalW, and the phylogenetic tree was generated by TreeView 1.5.2 (Page, 1996). The branch lengths represent numbers of substituted residues per site.