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Michaelis constants for substrates of recombinant N-methyltransferases
| Substrate |
Km
|
|||
|---|---|---|---|---|
| CaXMT1 | CaMXMT1 | CaMXMT2 | CaDXMT1 | |
| μm | ||||
| Methyl group acceptor | ||||
| XR | 78 ± 7 | |||
| 7mX | 148 ± 7 | 251 ± 20 | 916 ± 18 | |
| Px | 458 ± 36 | 738 ± 51 | 973 ± 88 | |
| Tb | 1,222 ± 197 | |||
| Methyl group donor | ||||
| AdoMet | 13 ± 2 | 12 ± 2 | 14 ± 1 | 153 ± 31 |
The values are the averages of the three independent measurements shown in Figure 4. Each value was obtained using Anemona (Hernandez and Ruiz, 1998).
