Figure 5.

Expression of MtGS2 during nodule development. A, Total RNA (15 μg lane⁻¹) isolated from roots and from nodules harvested from 5 to 20 d after inoculation with S. meliloti was fractionated on formaldehyde-agarose gels followed by RNA-blot hybridization using a ³²P-labeled GS2 fragment (MtGS2) and a rRNA gene fragment (rRNA). B, Total soluble protein extracted from similar roots and nodules (30 μg lane⁻¹) was fractionated by SDS-PAGE and transferred to nitrocellulose, and the membranes were probed with a GS antibody. GS1, GS2, and preGS2 designate cytosolic, plastid, and plastid precursor GS, respectively. Molecular masses are indicated.