Figure 7.

Localization of the GS2 precursor in nodule extracts fractionated by Percoll gradient centrifugation. Western immunodetection of GS, symbiosome membrane nodulin-26 (E6), and plastid membrane polypeptide (E37) in fractions of a nodule extract separated on a self-generated Percoll gradient. The fractions are numbered from the top to the bottom of the gradient. Equal amounts of total protein (50 μg) were loaded on a SDS-PAGE gel and transferred to nitrocellulose, and the membrane was probed with the different antibodies. Molecular mass markers are indicated.