Table I.
rat mutants
*, Mutant complemented with wild-type gene; **, attempted complementation failed; ***, kanamycin (kan) resistance does not cosegregate with rat phenotype; +, mutant scores less than 25% of wild-type; ++, mutant scores less than 33% of wild-type; +++, mutant scores less than 50% of wild-type; ++++, mutant scores more than 50% of wild-type but still a rat mutant; +++++, mutant scores at the level of wild-type for transient GUS activity; N/A, not applicable.
| Mutant | Identificationa | Collection | Tumorigenesis | Phosphinothricin (ppt) Resistance | Transient GUS | Zygosity | Resistance Marker | Gene Affected | Insertion Site |
|---|---|---|---|---|---|---|---|---|---|
| rat1* | F | Feldmann | + | + | + | homo | kan | Arabinogalactan protein | 5′-Untranslated region (UTR) |
| rat3* | F | Feldmann | + | + | +++ | homo | kan | Likely cell wall protein | Intergenic |
| rat4* | F | Feldmann | + | + | + | homo | kan | Cellulose synthase-like protein (CslA-09) | 3′-UTR |
| rat5* | F | Feldmann | + | + | +++++ | homo | kan | Histone H2A-1 | 3′-UTR |
| rat6 | F | Feldmann | + | + | + | kan | |||
| rat7 | F | Feldmann | + | + | + | kan | Unknown protein | ||
| rat8 | F | Feldmann | +++ | +++ | ++ | kan | |||
| rat9 | F | Feldmann | + | + | + | kan | Unknown protein | ||
| rat10 | F | Feldmann | + | + | ++ | kan | |||
| rat11 | F | Feldmann | + | + | ++ | kan | |||
| rat12 | F | Feldmann | + | + | + | kan | |||
| rat13 | F | Feldmann | + | + | + | kan | |||
| rat14 | F | Feldmann | + | + | ++ | kan | Unknown protein | 3′-UTR | |
| rat15 | F | Feldmann | + | + | + | kan | |||
| rat16 | F | Feldmann | + | +++ | ++ | kan | |||
| rat17*** | F | Feldmann | + | + | +++++ | kan | Myb transcription factor (cpc) | 3′-UTR | |
| rat18 | F | Feldmann | + | ++ | +++++ | kan | |||
| rat19 | F | Feldmann | + | + | + | kan | Intergenic | ||
| rat20 | F | Feldmann | + | +++ | +++++ | kan | |||
| rat21 | F | Feldmann | + | + | +++ | kan | |||
| rat22 | F | Feldmann | + | ++ | ++++ | kan | Unknown protein | Intergenic | |
| rat A1 | F | Feldmann | + | + | homo | kan | |||
| rat A2* | F, R | Feldmann | + | + | + | homo | kan | phosphatase 2A (rcn1) | Sixth exon |
| rat A3 | F | Feldmann | + | + | homo | kan | |||
| rat A4 | F | Feldmann | + | ++++ | homo | kan | Kinesin protein | First intron | |
| rat A5 | F | Feldmann | + | ++++ | homo | kan | Unknown protein | ||
| rat A6 | F | Feldmann | + | + | homo | kan | |||
| ratJ1* | F | Feldmann | + | ++ | + | homo | kan | Importin β-3 | 18th intron |
| ratJ2 | F | Feldmann | + | + | kan | MADS box protein | Fifth intron | ||
| ratJ3 | F | Feldmann | + | kan | |||||
| ratJ4 | F | Feldmann | + | ++ | kan | ||||
| ratJ5 | F | Feldmann | + | + | kan | ||||
| ratJ6 | F | Feldmann | + | + | kan | 3- Isopropylmalate dehydrogenase | Sixth exon | ||
| ratJ7 | F | Feldmann | + | + | homo | kan | DEAD box RNA helicase | Third intron | |
| ratJ8 | F | Feldmann | + | + | kan | ||||
| ratJ9 | F | Feldmann | + | + | kan | Mitochondrial chaperonin hsp60 | Fourth exon | ||
| ratJ10 | F | Feldmann | ++ | + | kan | ||||
| ratJ11 | F | Feldmann | + | + | kan | ||||
| ratJ12 | F | Feldmann | + | + | kan | ||||
| ratJ13 | F | Feldmann | + | + | kan | ||||
| ratJ14 | F | Feldmann | + | + | kan | ||||
| ratJR1 | F | Feldmann | + | + | kan | ||||
| ratJR2 | F | Feldmann | ++ | + | kan | ||||
| ratJR3 | F | Feldmann | + | + | kan | ||||
| ratJR4 | F | Feldmann | ++ | ++++ | kan | ||||
| ratJR5 | F | Feldmann | ++ | +++ | kan | ||||
| ratJR6 | F | Feldmann | ++++ | ++ | kan | ||||
| ratJR7 | F | Feldmann | + | + | + | kan | |||
| ratJR8 | F | Feldmann | + | ++ | ++ | kan | |||
| ratJR9 | F | Feldmann | +++ | + | ++++ | kan | |||
| ratJR10 | F | Feldmann | ++++ | + | ++++ | kan | |||
| ratJR11 | F | Feldmann | ++++ | + | + | kan | |||
| ratL1 | F | Bressan | +++ | ppt | Cyclin/cinnamoyl transferase | Third intron/3′-UTR | |||
| ratL2 | F | Bressan | +++ | ppt | |||||
| ratL3 | F | Bressan | +++ | ppt | ARID protein/METHF dehydrogenase | 5′-UTR/Fourth exon | |||
| ratL4 | F | Bressan | +++ | ppt | ATP citrate lyase/glucosidase | 5′-UTR | |||
| ratL5 | F | Bressan | + | ppt | A-T-rich repeat region | Intergenic | |||
| ratL6 | F | Bressan | ++ | ppt | Hypothetical protein | Exon | |||
| ratL7 | F | Bressan | + | ppt | CAAT repeat region | 3′-UTR | |||
| ratT2 | F | Feldmann | ++ | ++ | kan | ||||
| ratT3 | F | Feldmann | +++ | + | kan | rac GTPase-activating protein | 5′-UTR | ||
| ratT4 | F | Feldmann | + | + | kan | Ethylene-responsive element binding factor | 3′-UTR | ||
| ratT5* | F | Feldmann | + | + | ++ | homo | kan | DREB2A | Second exon |
| ratT6 | F | Feldmann | + | + | kan | ||||
| ratT7 | F | Feldmann | + | + | kan | ||||
| ratT8* | F | Feldmann | + | + | + | homo | kan | Receptor-like kinase | 3′-UTR |
| ratT9 | F | Feldmann | + | + | kan | Receptor-like kinase | 3′-UTR | ||
| ratT10 | F | Feldmann | + | + | kan | Unknown protein | 3′-UTR | ||
| ratT11 | F | Feldmann | + | + | kan | ||||
| ratT12 | F | Feldmann | + | ++ | kan | ||||
| ratT13 | F | Feldmann | + | + | kan | Unknown protein | 5′-UTR | ||
| ratT14 | F | Feldmann | ++ | +++ | kan | ||||
| ratT15 | F | Feldmann | + | + | kan | Unknown protein | 5′-UTR | ||
| ratT16* | F | Feldmann | + | + | + | homo | kan | Unknown protein | 6th intron |
| ratT17* | F | Feldmann | + | ++ | ++++ | homo | kan | Histone H3 | Intergenic |
| ratT18 | F | Feldmann | + | + | kan | β-Expansin | Intron | ||
| ratT19 | F | Feldmann | + | ||||||
| uta1* | F | Bressan | + | homo | ppt | Voltage-dependent anion channel | First exon | ||
| uta2 | F | Bressan | + | homo | ppt | F-box protein | Exon | ||
| act2-1 | R | Feldmann | + | + | ++ | homo | kan | Actin-2 (root actin) | First intron |
| act7-4* | R | Feldmann | + | + | +++ | homo | kan | Actin-7 (root actin) | First intron |
| act7-1* | R | Feldmann | + | +++ | +++ | homo | kan | Actin-7 (root actin) | Fourth intron |
| Importin α-7* | R | Feldmann | + | ++ | +++ | homo | kan | Importin α-7 | Seventh intron |
| ratH1 | R | Feldmann | + | + | +++ | kan | Unknown protein | Intergenic | |
| cep1 | R | Feldmann | + | + | homo | kan | Constitutive expression of PR1,2,5 genes | ||
| atrx1* | R | Feldmann | + | homo | kan | Atrx1 | |||
| rad5 | R | + | ++++ | ++ | Point mutation | ||||
| HTA2 | R | Feldmann | ++ | homo | kan | Histone H2A-2 | 5′-UTR | ||
| HTA3 | R | Feldmann | + | homo | kan | Histone H2A-3 | 3′-UTR | ||
| HTA10 | R | Feldmann | ++++ | +++++ | homo | kan | Histone H2A-10 | 3′-UTR | |
| HTA11 | R | Feldmann | + | homo | kan | Histone H2A-11 | 3′-UTR | ||
| HTA13 | R | Feldmann | ++++ | +++++ | homo | kan | Histone H2A-13 | 5′-UTR | |
| HTB5 | R | Feldmann | ++ | homo | kan | Histone H2B-5 | 5′-UTR | ||
| HTB6 | R | Feldmann | ++ | +++++ | homo | kan | Histone H2B-6 | 3′-UTR | |
| HTR4/5 | F, R | Feldmann | +++ | homo | kan | Histone H3-4/5 | Intergenic | ||
| HFO3 | R | Feldmann | + | homo | kan | Histone H4-3 | 3′-UTR | ||
| HFO4 | R | Feldmann | + | +++++ | homo | kan | Histone H4-4 | 3′-UTR | |
| HAT6 | R | Feldmann | + | + | +++ | homo | kan | Histone acetyl transferase-6 | 5′-UTR |
| HAC11 | R | Feldmann | ++ | +++ | homo | kan | Histone acetyl transferase-11 | 3′-UTR | |
| HDA1 | R | Feldmann | + | +++++ | homo | kan | Histone deacetylase-1 | Exon | |
| HDA2 | R | Feldmann | ++++ | +++++ | homo | kan | Histone deacetylase-2 | Exon | |
| HDA6 | R | Feldmann | +++ | homo | kan | Histone deacetylase-6 | 3′-UTR | ||
| HDA9 | R | Feldmann | ++++ | homo | kan | Histone deacetylase-9 | 3′-UTR | ||
| HXA1 | R | Feldmann | +++ | + | hetero | kan | Histone acetylase complex HXA1 | 5′-UTR | |
| HXA2 | R | Feldmann | + | ++++ | kan | Component ADA2 homolog HXA2 | 5′-UTR | ||
| CS2491 | R | Feldmann | + | homo | kan | Disease resistance gene | |||
| Unknown (HAT4) | R | Feldmann | + | homo | kan | Homologous to phytoene hydroxylase | |||
| RNAi CHA 6A | R | ++++ | hyg | Chromatin-remodeling complex subunit 6 | N/A | ||||
| RNAi CHA 6C | R | ++++ | hyg | Chromatin- remodeling complex subunit 6 | N/A | ||||
| RNAi HAC8 | R | Line 156 A | + | homo | hyg | Histone acetyl transferase-8 | N/A | ||
| R | Line 156 B | + | homo | hyg | Histone acetyl transferase-8 | N/A | |||
| RNAi NFA2 | R | Line 300 A | +++ | homo | hyg | Nucleosome assembly factor A | N/A | ||
| R | Line 422 A | ++++ | homo | hyg | Nucleosome assembly factor A | N/A | |||
| RNAi SGA1 | R | Line 524 A | ++++ | homo | hyg | Chromatin-silencing group 1 | N/A | ||
| R | Line 524 B | + | homo | hyg | Chromatin-silencing group 1 | N/A | |||
| RNAi BTI1 | R | Line 23 | + | + | ppt | Unknown protein | N/A | ||
| RNAi BTI2 | R | Line 45 | + | + | ppt | Unknown protein | N/A | ||
| RNAi BTI3 | R | Line 16 | + | + | ppt | Unknown protein | N/A | ||
| RNAi AtRAB8 | R | Line 22 | + | + | ppt | AtRAB8 | N/A | ||
| rat4 Antisense lines | R | Line L | +++ | hyg | CsIA-09 | N/A | |||
| R | Line M | ++++ | hyg | CsIA-09 | N/A | ||||
| R | Line N | ++++ | hyg | CsIA-09 | N/A | ||||
| Importin α-1 antisense lines | R | Line 1 | +++ | +++ | ppt | Importin α-1 | N/A | ||
| R | Line 2 | ++++ | ++++ | ppt | Importin α-1 | N/A | |||
| R | Line 3 | ++ | ++ | ppt | Importin α-1 | N/A | |||
| R | Line 4 | +++ | ++ | ppt | Importin α-1 | N/A | |||
| BTI1 antisense | R | Line 132 | ++ | + | + | kan | Unknown protein | N/A | |
| BTI2 antisense | R | Line 107 | + | +++ | + | kan | Unknown protein | N/A | |
| RAB8 antisense | R | Line 19 | + | +++ | +++ | kan | AtRAB8 | N/A | |
| VIP1 antisense | R | (In tobacco [Nicotiana tabacum]) | + | kan | VirE2-interacting protein | N/A |
a
Mutant identified by forward (F) or reverse (R) genetics