Table II.
Steps of the transformation process putatively disrupted in selected Arabidopsis rat mutants +, Mutant scores less than 25% of the wild-type; ++, mutant scores less than 33% of the wild-type; +++, mutant scores less than 50% of the wild-type; ++++, mutant scores more than 50% of the wild-type but still a rat mutant; +++++, mutant scores at the level of wild-type for transient GUS activity; *, mutant has been complemented with the wild-type gene; ND, not determined.
| Mutant | Tumorigenesis | Transient GUS | Gene Affected |
|---|---|---|---|
| Bacterial attachment/T-DNA transfer | |||
| rat1* | + | + | Arabinogalactan protein |
| rat3* | + | +++ | Likely cell wall protein |
| rat4* | + | + | AtCslA-09 |
| ratT18 | + | ND | β-Expansin |
| Antisense rat4 | +++ | ND | AtCslA-09 |
| Antisense F9 | ++ | + | Unknown protein |
| Antisense F8 | + | + | Unknown protein |
| Antisense RAB8 | + | +++ | AtRAB8 |
| RNAi BTI1 | + | + | Unknown protein |
| RNAi BTI2 | + | + | Unknown protein |
| RNAi BTI3 | + | + | Unknown protein |
| RNAi AtRAB8 | + | + | AtRAB8 |
| Cytoplasmic trafficking/cytoskeleton | |||
| act2-1 | + | ++ | Actin-2 |
| act7-4* | + | +++ | Actin-7 |
| act7-1* | + | +++ | Actin-7 |
| rat A4 | + | ND | Kinesin protein |
| Nuclear targeting | |||
| ratj1* | + | + | Importin β-3 |
| Importin α-7* | + | +++ | Importin α-7 |
| Antisense importin α-1 | ++/+++ | ++/+++ | Importin α-1 |
| T-DNA integration/chromatin structure and remodeling | |||
| rat5* | + | ++++ | Histone H2A-1 |
| HTA2 | ++ | ND | Histone H2A-2 |
| HTA3 | + | ND | Histone H2A-3 |
| HTA10 | ++++ | +++++ | Histone H2A-10 |
| HTA11 | + | ND | Histone H2A-11 |
| HTA13 | ++++ | +++++ | Histone H2A-13 |
| HTB5 | ++ | ND | Histone H2B-5 |
| HTB6 | ++ | +++++ | Histone H2B-6 |
| HTR4/5 | +++ | ND | Histone H3-4/5 |
| HFO3 | + | ND | Histone H4-3 |
| HFO4 | + | +++++ | Histone H4-4 |
| HDA1 | + | +++++ | Histone deacetylase-1 |
| HDA2 | ++++ | +++++ | Histone deacetylase-2 |
| HDA6 | +++ | ND | Histone deacetylase-6 |
| HDA9 | ++++ | ND | Histone deacetylase-9 |
| HAT6 | + | +++ | Histone acetyl transferase-6 |
| HAC11 | ++ | + | Histone acetyl transferase-11 |
| HXA1 | +++ | + | Histone acetylase complex HXA1 |
| HXA2 | + | ++++ | Histone acetylase complex HXA2 |
| RNAi CHA6 | ++++ | ND | Chromatin-remodeling complex subunit 6 |
| RNAi HAC 8-1 | + | ND | Histone acetyl transferase-8 |
| RNAi NFA2-1 | +++ | ND | Nucleosome assembly factor A |
| RNAi SGA1 | + | ND | Chromatin-silencing group 1 |