Table V.

Comparison of expression patterns of 20 well-known cold responsive genes between pollen and leaf

		SAGE (Tag Counts)				Microarray (Ratio)
Gene Products	Tag_Sequence	Pollen		Leafa		Pollen C/N	Leaf C/N
		N	C	N	C
Cold-responsive genes
Chalcone synthase	TCGAGCGCGT	0	0	0	80	1.17	2.80
pyrroline-5-carboxylate synthetase A	CTCGTGGTCC	0	0	1	21	0.91	13.72
β -Amylase enzyme (ct-bmy gene)	GGAGGAGACT	0	0	0	144	1.33	4.68
Alcohol dehydrogenase	GGTGCTTGAA	0	0	0	28	1.37	19.33
Lipid transfer protein 3	TATTTGTTTT	0	0	1	38	1.40	4.50
Lipid transfer protein 4	TTTAAGATAT	0	0	0	17	1.09	52.22
Fatty acid desaturase/cytochrome b5 fusion protein	GAAGAGATGT	6	36	0	19	0.87	8.21
Cold-regulated protein cor15a precursor	TTTAATAGTA	0	0	0	389	5.46	133.04
Early light-induced protein	TGTACTAAGT	0	0	0	203	1.20	19.46
Iti30	AAAATAAAAG	0	0	0	104	2.09	305.80
Early light-inducable protein	ACTTCAGACG	0	0	0	80	1.52	149.94
cDNA 5′ similar to dehydrin erd10 (low-temperature-induced protein Iti45)	CCAGCACCAC	0	0	3	105	1.53	8.95
Gly-rich protein (RAB18)	AGTGTGTAAT	0	0	0	30	1.24	25.76
Cold acclimation protein WCOR413-like protein α -form	TAAGAGTGAT	0	0	0	42	1.42	12.18
cor15b	TTCAATAGTT	0	0	0	19	1.22	8.37
rd22	TTGTGTGGTT	0	0	4	75	0.98	8.12
Senescence-associated protein (SAG29)	AGGATCTGTC	0	0	0	13	1.23	23.86
Putative dessication-related protein LEA1	ACGGCTCTTG	0	0	3	63	1.52	10.98
LEA D113 type1 protein	CAATGTAACA	0	0	0	16	0.81	38.50
FL3-5A3 mRNA for cold acclimation protein homolog	AGGTGTTAGT	0	0	3	27	0.78	8.90
Housekeeping genes
α -Tubulin	TATGCGAAGA	0	0	3	2	0.97	0.84
Actin8	AAGATTAAGG	3	2	1	2	1.03	0.92
Ubiquitin-conjugating enzymeE2	GGCTGACACA	19	6	3	11	0.54	1.25

^aData from Jung et al. (2003); N, normal; C, cold.