Table I.
Analysis of total leaf lipids and MGDG in wild-type and T2 generation LMIA leaf tissue
Lipids were extracted from 100 mg of leaf tissue, and a portion was removed and derivatized for analysis of total lipid content as described in “Materials and Methods.” Lipids were spotted on NH4SO4-impregnated silica gel plates and developed in acetone:toluene:water (91:30:8 [v/v]). Spots corresponding to known lipid standards were scraped from the plates and derivatized after addition of appropriate amount of standard. Fatty acids were analyzed by GC as described in the text.
| Plant Line | Wild Type (n = 4)a | LMIA-3 (n = 3) | LMIA-16 (n = 3) | LMIA-17 (n = 3) | LMIA-18 (n = 2) | LMIA-25 (n = 3) | Average LMIA |
|---|---|---|---|---|---|---|---|
| Leaf lipid content (μg mg fresh wt-1) | 5.1 ± 0.8 | 3.7 ± 0.5b | 3.7 ± 0.9b | 3.2 ± 0.1b | 3.3 ± 0.8b | 4.0 ± 0.6 | 3.6 ± 0.6b |
| % 16:3 (mol % of total) | 12.0 ± 0.7 | 7.7 ± 1.2b | 8.4 ± 2.8b | 7.0 ± 2.2b | 7.9 ± 2.6b | 6.5 ± 1.4b | 7.5 ± 1.9b |
| C18:C16c | 2.9 ± 0.1 | 3.5 ± 0.2b | 3.6 ± 0.5b | 3.9 ± 0.3b | 3.8 ± 0.5b | 4.1 ± 0.1b | 3.8 ± 0.4b |
| MGDG (% total leaf lipids) | 53.7 ± 10.1 | 53.3 ± 9.5 | 41.4 ± 3.3 | 49.3 ± 19.5 | 30.2 ± 0.3b | 36.9 ± 10.5 | 43.1 ± 12.6 |
| % 16:3 in MGDG | 27.4 ± 1.3 | 18.1 ± 4.3b | 19.4 ± 3.3b | 14.6 ± 1.7b | 18.1 ± 5.1b | 16.6 ± 1.9b | 17.3 ± 3.2b |
a
n = No. of plants sampled from each line. b Significantly different from wild-type values (P < 0.05). c Ratio of C18 to C16 fatty acids in total leaf lipid extract.