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. 2003 Jun;132(2):768–778. doi: 10.1104/pp.102.016642

Table I.

Side-chain analysis of xyloglucan derived from various Arabidopsis tissues

XyG oligosaccharides from the various tissues were characterized by 1H-NMR spectroscopy (see “Materials and Methods”). Data were normalized by setting the total number of side chains per oligosaccharide subunit to 3, consistent with the “XXXG-type” structure (Vincken et al., 1997) of Arabidopsis XyG. O-Acetyl substituents are hydrolyzed by 4 N KOH treatment.

Tissue
Side Chains Per Subunit (Percentage of Side Chains with Acetatylated Gal Residue)
Fuc→Gal→Xyl Gal→Xyl Xyl
XEG-extracted xyoglucan
    Flowers 0.51a 0.31a 2.08
    Rosettes 0.40a 0.60a 2.00
    Top stem 0.43 (22) 0.40 (14) 2.17
    Lower stem 0.43 (29) 0.50 (18) 2.07
    Siliques 0.44a 0.52a 2.04
    Roots 0.47a 0.48a 2.05
KOH-extracted xyoglucan
    Flowers 0.46 0.41 2.13
    Rosettes 0.53 0.51 1.96
    Top stem 0.48 0.39 2.13
    Lower stem 0.55 0.41 2.04
    Siliques 0.50 0.47 2.03
    Roots 0.49 0.27 2.24
    Cauline 0.50 0.50 2.00
a

The indicated XEG-extracted samples did not contain sufficient material to accurately quantitate the levels of O-acetylation. However, the data clearly indicated that for all XEG-extracted samples in this table, less than 45% of the Fuc→Gal→Xyl side chains and less than 20% of the Gal→Xyl side chains contained an O-acetylated Gal residue. The highest levels of O-acetylation were found in rosettes, lower stem, and siliques.