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. 2003 Jun;132(2):786–795. doi: 10.1104/pp.102.019331

Figure 4.

Figure 4.

Overexpression of the fra5 and fra6 mutant cDNAs in transgenic plants. Five-day-old seedlings of the wild-type (WT) and transgenic plants with overexpression of the fra5 mutant cDNA (35S::fra5) or the fra6 mutant cDNA (35S::fra6) were used for RNA isolation and subsequent RT-PCR analysis. Expression of a ubiquitin gene was used as an internal control. A, Gene-specific primers as described in “Materials and Methods” were used to determine the level of the fra5 mutant mRNA (fra5), that of the endogenous AtCesA7 mRNA (CesA7), and the combined level of the fra5 mutant mRNA and the endogenous AtCesA7 mRNA (fra5+CesA7). Note that although a high level of the fra5 mRNA was seen in the 35S::fra5 lines, the amount of the endogenous AtCesA7 mRNA was not altered. B, Gene-specific primers were used to determine the level of the fra6 mutant mRNA (fra6) and the combined level of the fra6 mutant mRNA and the endogenous AtCesA8 mRNA (fra6+CesA8).