Figure 4.

Decrease of anthocyanin in young seedlings of 35S-ASN1 lines under high Suc and low nitrogen conditions. Anthocyanin contents (A₅₃₀ - 0.25A₆₅₇) of 10-d-old seedlings grown on nitrogen-free Murashige and Skoog agar plates containing 3% (w/v) Suc and 0.5 mm Gln were measured using a spectrometric method as described in “Materials and Methods.” Each data point represents an average value determined from four pools of 36 seedlings for each line. Seeds of different lines were sown onto the same agar plate to ensure homogeneity as described in “Materials and Methods.” Error bars = ses. The data were analyzed by one-way ANOVA followed by lsd test. ^**, Corresponding lines were grouped distinctly from both control lines with P values less than 0.01. FW, Fresh weight.