Figure 7.

Elevation of free Asn levels in phloem exudates of 35S-ASN1 lines. Plants were grown for 5 weeks under regular day/night cycle, and individuals that bore at least 10 to 15 developing siliques were used in the analysis. Phloem exudates were obtained using a scaled-down EDTA elution method, and free amino acid contents were determined as described in “Materials and Methods.” Each bar represents an average of three samples. Error bars = ses. The data were analyzed by one-way ANOVA followed by lsd test. ^**, Corresponding lines were grouped distinctly from both control lines with P values less than 0.01. DW, Dry weight after phloem exudate collection.