Figure 2.

Relative expression level and transcript abundance of fermentation genes in aerobic conditions during development. A, Quantification of mRNA levels was achieved by using a quantitative real-time PCR system (see “Materials and Methods”). ADH and PDC expression levels were normalized with respect to the internal control ACT2 and are plotted relative to the expression from whole seedlings. Data bars represent the mean ± se level of transcripts from three experiments with independent RNA extractions. Note different scales as y axis. B, PDC transcript abundance in different organs. Total PDC mRNA in seedlings = 100. Contribution from individual genes according to A is represented.