Figure 3.

Relative expression level and transcript abundance of fermentation genes during anoxia. A, RNA gel-blot analysis from seedlings treated with different periods of anoxia. Hybridization probes specific to each of the PDC genes were used. 25S hybridization was used as control. B, Quantification of ADH and PDC transcripts from seedlings submitted to 24-h anoxia using the a quantitative real-time PCR system. Expression of RD29a was used as a control. Expression levels were normalized with respect to the internal control ACT2 and are plotted relative to the expression at 0-h anoxia. Data bars represent the mean ± se level of transcripts from three experiments with independent RNA extractions. Note different scales as y axis. C, PDC transcript abundance in anoxia-treated seedlings. Contribution from individual genes according to A is represented.