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. 2003 Jul;132(3):1217–1227. doi: 10.1104/pp.102.019026

Figure 1.

Figure 1.

Expression of cell division and meristematic-associated markers in the pas mutants. (A) CDKA::GUS expression in wild-type, pas1, pas2, and pas3 seedlings (from left to right, two seedlings each) 10 d after germination. Wild-type seedlings were grown in the absence (left) or presence of 0.1 μm 6-benzyladenine (BA; right). All of the pas mutants were grown in absence of cytokinins. (B) CYCB1::GUS expression in wild-type, pas1, pas2, and pas3 seedlings (from left to right, two seedlings each) 10 d after germination. CDKA and CYCB1 were strongly expressed in the meristems and were ectopically expressed in the leaves of the pas mutants. (C) CYCB1::GUS expression in pas1 mutant 10 d after germination showing localized area of ectopic CYCB1 expression in the leaves. (D) CycB1::db::GUS expression in 5 d after germination wild-type root. (E) CycB1::db::GUS expression 5 d after germination in pas2-1 root. (F) CycB1::db::GUS expression 5 d after germination in wild-type seedling. (G and H) CycB1::db::GUS expression 5 d after germination in pas2-1 seedling. (I) KNAT2::GUS expression in wild-type, pas1, pas2, and pas3 seedlings (from left to right, two seedlings each) 10 d after germination. Wild-type seedlings were grown in the absence (left) or presence of 0.1 μm BA (right). The corresponding meristematic areas are enlarged, showing increased expression of KNAT2 in the presence of cytokinins (right insert) compared with control (left insert). The pas mutants were grown in the absence of cytokinins. (J) KNAT2::GUS expression in the pas2 mutant 18 d after germination. (K) KNAT2::GUS expression in the pas3 mutant 10 d after germination. KNAT2::GUS staining shows the existence of enlarged or doubled meristem in the pas mutants. (L) KNAT2::GUS expression in mature wild-type embryo. (M) KNAT2::GUS expression in mature pas2 embryo. Scale bar represents 1 mm except for D, E, L, and M, where it represents 200 μm.