Figure 4.

Multiple sequence alignment of ElLTP1 and ElLTP2 with MS/MS acquired peptides of the putative ElLTP3. Black boxes indicate that identical amino acids are present in at least two-thirds of the sequences compared, whereas gray boxes indicate that amino acids with similar physiochemical properties are present in two-thirds of the compared sequences. Peptides sequenced obtained in MS/MS analysis of ElLTP1 and ElLTP2 are underlined. The presumptive N terminus of the mature ElLTP1 is indicated by an asterisk. It should be noted that when sequencing with MS/MS, it is not possible to tell the difference between residues of equal molecular mass, like Leu (L) and Ile (I) or Phe (F) and oxidated Met (M). ElLTP1 and ElLTP2 have the accession numbers AAM00272 and AAM00273 in the National Center for Biotechnology Information database.