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Interaction between CDKB2;1 and CYCD4;1 in yeast. Yeast strain Y190 was transformed with different combinations of pAS-CDKB2;1, pACT-CYCD4;1, or the empty vector pAS2–1 or pACT2. As a positive control, pTD1–1 carrying SV40 large T antigen and pVA3–1 carrying murine p53 were transformed. β-Galactosidase activity of yeast cells grown on a minimal medium was observed by the colony filter assay.
