Arabidopsis cyclins interacting with CDKB2;1. In vitro pull-down assay was
conducted with D-type and mitotic cyclins. A, [35S]Met-labeled
CYCD1;1, CYCD2;1, CYCD3;1, and CYCD4;1 were incubated with GST (lane 2) or
GST-CDKB2;1 (lane 3) immobilized on glutathione Sepharose beads. Proteins
bound to the beads were separated by SDS-PAGE and autoradiographed.
One-microliter input of the translation products is included as a control
(lane 1). Arrowheads indicate the position of in vitro translated cyclins. B,
CYCA2;2 and CYCB2;1 were incubated with the beads as described above.