Figure 3.

Western-blot analysis of crude extracts from different leaves of a single offshoot from clone EF 3. A, Extracts from leaf pairs 1 to 7 from an erect (flowering) stem were loaded in lanes 2 to 8, respectively. Lane 1 was loaded with 0.2 μg of purified Gleheda. B, Extracts from leaf pairs 1 to 9 from a (vegetatively growing) creeping offshoot were loaded in lanes 2 through 10, respectively. Lane 1 was loaded with 0.2 μg of purified Gleheda. All samples were reduced with 2% (v/v) β-mercaptoethanol. The oldest leaves of both the erect and creeping stem were approximately 25 d old.