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. 2003 Jul;132(3):1322–1334. doi: 10.1104/pp.103.023853

Figure 5.

Figure 5.

Immunofluorescence labeling of Gleheda in leaves of ground ivy. Developing leaves (A–D) and fully developed leaves (E–H) were used for embedding. Cross sections of leaves of a no-lectin (clone CA 1; A and E) and a highlectin plant (clone EF 5; B–D and F–H) were probed with anti-Gleheda-antibody, followed by a labeling with fluorescence-labeled secondary antibody. Labeling in the sections of the low-lectin clone exhibits only the yellow-brown autofluorescence of chloroplasts (A and E), whereas the strong green fluorescence label within the palisade parenchyma in the sections of the high-lectin clone (B and F) indicates the location of the lectin. Higher magnifications of B and F are shown in C and G, respectively, to visualize the subcellular location of Gleheda. Note the high amount of fluorescence-labeled Gleheda within the vacuoles (v). DNA-containing organelles (n, nucleus; p, plastids) were identified by concomitant 4,6-diamidino-2-phenylindole (DAPI) staining (shown in D and H). Bars = 50 μm in A, B, E, and F; and 10 μm in C, D, G, and H, respectively.