PCR analysis of control and putative transformants. A, PCR products (1.65
kb) using 3P/3M primers show integration into the chloroplast genome. Lane 1,
Marker; lane 2, pLDR-MerAB transgenic line; lane 3, pLDR-MerAB-3′-UTR
transgenic line; lane 4, untransformed wild type. B, PCR products (3.8 kb)
using 5P/2M primers confirm merAB integration. Lane 1, Marker; lane
2, pLDR-MerAB transgenic line; lane 3, pLDR-MerAB-3′-UTR transgenic
line; lane 4, positive control (pLDR-MerAB plasmid DNA); lane 5, untransformed
wild-type tobacco.