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Oligonucleotides sets used to construct the synthetic genes encoding: A, AP-enhanced green fluorescent protein (EGFP); B, TP-EGFP; and C, VP-EGFP. Internal repeat oligonucleotide sets were polymerized head-to-tail in the presence of the 5′-linker sets. After ligation, the 3′ linker sets were added. The restriction sites (underlined) were used to subclone the genes into pUC18 (BamHI-EcoRI), then into pUC-SStom-SP-EGFP in place of the SP gene (XmaI-NcoI).
