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. 2003 Jul;132(3):1370–1381. doi: 10.1104/pp.103.022970

Figure 4.

Figure 4.

Time course of CPN1 transcript accumulation after bacterial inoculation. A, RNA gel-blot analysis of the time course of CPN1 transcript accumulation after inoculation with avirulent P. s. t. DC3000 (avrRpt2) bacteria. Leaves of 5-week-old WT plants were syringe inoculated with 1 × 105 cfu mL–1 of P. s. t. DC3000 (avrRpt2) bacteria, and RNA samples were extracted from inoculated leaves at 0, 2, 4, 6, 9, 12, 24, 36, 48, and 72 h postinoculation. PR1, Same blot reprobed with a PR1 gene-specific probe. Mock, RNA samples from leaves inoculated with 10 mm MgCl2 at 0 and 24 h postinoculation. rRNA, 28S rRNA stained with methylene blue to show relative amounts of RNA in each lane. B, RNA gel-blot analysis of the time course of CPN1 transcript accumulation after inoculation with virulent P. s. t. DC3000 bacteria. This experiment was performed in a manner identical to that described in A.