Analysis 5′-UTR-deleted transgenic GUS reporter lines. A, RT-PCR
analyses of GUS transcript levels of six independent lines. C, Control; S,
salinity stress (4 h, 200 mm NaCl soil saturation). B, Four lines
varying in GUS activity were subjected to 4 h of salt treatment by soil
saturation with 200 mm NaCl. In a parallel experiment, wounding was
applied to salt-stressed plants by cutting (three leaves of five plants per
line). The leaf material at the vicinity of the wounding site was harvested
and pooled per line. The error bars are based on two independent
experiments.