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Dependence of the guaiacol peroxidase activity of partially purified pmPOX on pH. The rates of guaiacol oxidation were determined under the standard assay conditions except that 25 mm sodium acetate (pH 4.0–5.0), MES (pH 5.5–6.5), or HEPES (pH 7.0–8.0) buffers were used. Data presented are average values ± sd of n = 3 experiments. ▪, pmPOX1; ○, pmPOX2.
