Table II.
The effects of lectins on guaiacol-dependent peroxidase activity of POX
Guaiacol-dependent peroxidase activity was measured at pH 5.0 after cation exchange chromatography. Fractions were measured in absence or presence of different lectins. Pre-incubation was for 3 min. Data are given as mean ± sd (n). For control rates, see Table I.
| Substance | Concentration | pmPOX1 | pmPOX2 | wPOX1 | wPOX2 |
|---|---|---|---|---|---|
| μg mL-1 | % of control | ||||
| Control | 100.0 ± 1.5 (3) | 100.0 ± 4.9 (3) | 100.0 ± 2.3 (3) | 100.0 ± 0.3 (3) | |
| Con A | 1 | 79.8 ± 3.3 (4) | 79.8 ± 2.0 (4) | 97.9 ± 5.2 (3) | 98.4 ± 3.6 (3) |
| 5 | n.m.a | 78.1 ± 2.8 (3) | 111.5 ± 1.9 (3) | 90.5 ± 4.1 (3) | |
| WGA | 1 | 78.2 ± 2.4 (3) | 85.6 ± 1.9 (4) | 106.1 ± 9.0 (3) | 90.4 ± 1.9 (3) |
| 5 | 81.9 ± 4.9 (3) | n.m. | 88.8 ± 2.6 (3) | 83.6 ± 3.2 (3) | |
a
n.m., Not measured.