Changes in photosynthetic pigments during chloroplast differentiation of
etiolated seedlings upon exposure to continuous white light of different
intensities. A, Changes in total carotenoid content. B, Changes in total
chlorophyll content. Pigments were extracted from 30 seedlings per time point.
Pigment content was determined spectroscopically in acetone. For chlorophylls,
the quantification was also done after separation on HPLC. Values are means
± sd of six independent determinations (except for 12 h,
n = 4, and 48 h, n = 3). ET, Etiolated seedlings; FW, fresh
weight.