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. 2003 Jul;132(3):1508–1517. doi: 10.1104/pp.102.019364

Figure 4.

Figure 4.

A, Northern-blot analysis of the expression of genes involved in pigment synthesis and light harvesting during chloroplast differentiation of tobacco seedlings under various light intensities. Four-day-old etiolated seedlings were exposed to continuous white light of LL (10 μmol m–2 s–1; gray box) or ML (100 μmol m–2 s–1; white box) intensity for different time periods (0, 3, 5, 8, 12, and 24 h). Gel-blot analysis was performed after separation of total RNA extracted from the individual samples (minimum of 60 seedlings each). For hybridization, nonradioactively labeled DNA fragments of the various xanthophyll biosynthetic genes were used as probes. bhy, β-carotene hydroxylase; zep, zeaxanthin epoxidase; vde, violaxanthin de-epoxidase; cab, chlorophyll a/b binding protein of LHC II; and ET, etiolated seedlings. Bottom, 25S rRNA hybridization for verification of gel loading. B, Densitometric scan of mRNA patterns of the β-carotene gene during chloroplast differentiation of tobacco seedlings. Signals were normalized with respect to the rRNA. The value for etiolated seedlings was set to 1. n = 3.