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Effect of the photosynthetic inhibitors DCMU (A) and DBMIB (B) on expression levels of various carotenoid biosynthetic genes. Three-day-old fully greened seedlings were treated with 2 mm DCMU or 50 μm DBMIB for3hinthe dark or in the light, respectively. Untreated seedlings served as control (–DCMU and –DBMIB). bhy, β-Carotene hydroxylase; zep, zeaxanthin epoxidase; and vde, violaxanthin de-epoxidase. Bottom, Hybridization with 25S rRNA for loading verification.
