Effect of the photosynthetic inhibitors DCMU (A) and DBMIB (B) on
expression levels of various carotenoid biosynthetic genes. Three-day-old
fully greened seedlings were treated with 2 mm DCMU or 50
μm DBMIB for3hinthe dark or in the light, respectively.
Untreated seedlings served as control (–DCMU and –DBMIB).
bhy, β-Carotene hydroxylase; zep, zeaxanthin epoxidase;
and vde, violaxanthin de-epoxidase. Bottom, Hybridization with 25S
rRNA for loading verification.