LeSNF4 encodes a γ-subunit homolog of the SnRK1 complex. A, Predicted
amino acid sequences of tomato LeSNF4 (AF143742), bean Pv42 (U40713),
Arabidopsis predicted protein (AAD39660), AKINγ (P80385), AtSNF4
(AF250335), the γ-domain (amino acids 125–497) of maize
ZmAKINβγ-1 (AF276085), and yeast SNF4 (172636) were aligned using
the Clustal method with PAM250 residue weight (DNASTAR Inc., Madison, WI).
Shading indicates extent of conservation/similarity. In addition to direct
sequence homology, CBS motifs based upon protein structure
(http://www.sanger.ac.uk/cgi-bin/Pfam/)
are shared between SNF4 (underlined) and LeSNF4 (overlined). B, Phylogenetic
tree of protein sequences shown in A based upon Clustal analysis (DNAStar
Inc.).