Figure 6.

Expression of LeSNF4 mRNA in gib-1 tomato seeds and wild-type tissues. Seeds were imbibed for the indicated time in either water or 100 μm GA₄₊₇ at 25°C before dissecting into the endosperm caps (endosperm tissue covering the radicle tip), radicle tips, and the remainder of the seed (for diagram, see Cooley et al., 1999). In addition, flower (F), leaf (L), and root (R) tissues were collected from field-grown wild-type (cv Moneymaker) plants. Extracted total RNAs were hybridized to riboprobes prepared from LeSNF4 (upper) or from a cDNA fragment (G46) having homology to constitutively expressed ribosomal protein sequences to confirm equal RNA loading of each lane (lower).