Figure 7.

Expression of SnRK1-related genes during tomato seed development. A, Fresh and dry weights and water contents (fresh weight basis) of tomato seeds during development. B, Abundance of LeSNF4, LeSNF1, and LeGAL83 mRNAs during tomato seed development. Total RNA was extracted from freshly harvested seeds at the times indicated and hybridized with probes prepared from the different genes (StubGAL83 probe was used to detect LeGAL83). Lower panels in each pair labeled rRNA are ethidium bromide-stained gels showing loading of RNA in each lane. C, Relative mRNA abundance was quantified by densitometry from the images in B and normalized for RNA loading in each lane. Within each panel, the normalized signal intensity at 60 d after flowering (DAF) was set equal to 1, and the normalized signal intensities in other lanes are shown relative to it.