Table I.
Two-hybrid analyses of protein-protein interactions among tomato and yeast SnRK1 proteins
| Binding Domain (Bait) | Activation Domain (Prey) | Galactosidase Activity ± SEa | ||
|---|---|---|---|---|
| High Glc (R) | Low Glc (D) | D/Rb | ||
| nmol min-1 g fresh wt-1 | ||||
| SNF4 | SNF1 | 64.2 ± 5.8 | 300 ± 23 | 4.7 |
| LeSNF4 | LeSNF1 | 2.79 ± 0.20 | 52.3 ± 5.9 | 19 |
| LeSIP1 | LeSNF1 | 235 ± 32 | 1,880 ± 220 | 8.0 |
| LeSIP1 | LeSNF4 | 222 ± 11 | 1,670 ± 284 | 7.5 |
| LeSNF1 | SNF4 | 6.25 ± 0.82 | 392 ± 75 | 63 |
| LeSNF4 | SNF1 | 1.94 ± 0.60 | 47.3 ± 5.9 | 24 |
| LeSIP1 | SNF1 | 17.3 ± 1.4 | 46.4 ± 2.0 | 2.7 |
| LeSIP1 | SNF4 | 6.27 ± 1.05 | 21.0 ± 5.0 | 3.4 |
| LeSNF1 | LeSNF1 | 1.59 ± 0.31 | 62.7 ± 28.5 | 39 |
| LeSNF4 | LeSNF4 | NDc | 4.56 ± 1.11 | - |
| LeSIP1 | LeSIP1 | 18.9 ± 4.0 | 84.1 ± 19.1 | 4.4 |
| LeSNF1 | Empty | 6.89 ± 0.82 | NDc | - |
| Empty | LeSNF1 | 5.13 ± 1.57 | NDc | - |
| LeSNF4 | Empty | 5.32 ± 2.23 | NDc | - |
| Empty | LeSNF4 | 5.93 ± 1.54 | NDc | - |
| LeSIP1 | Empty | 3.44 ± 2.29 | NDc | - |
| Empty | LeSIP1 | NDc | NDc | - |
a
Yeast 190 cells transformed with different coding regions in the binding or activation domains of the yeast two-hybrid vector were grown in SC-Trp-Leu-His plus 2% (w/v) Glc (high Glc [R = repressed]) or 2% (w/v) each of galactose/glycerol/ethanol plus 0.05% (w/v) Glc (low Glc [D = derepressed]) broth containing 25 mM 3-amino-triazole. β-Galactosidase activities in crude protein extracts from three or four yeast colonies were measured as described in “Materials and Methods.” n = 3 or 4. b D/R = value in low Glc (D) divided by value in high Glc (R). c ND, Not detected.