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PID interacts with PBP1 and TCH3 in yeast. Yeast cells containing pACT-PBP1 or pACT-TCH3 and pAS2-1-PID (lanes 1 and 4), pAS2-1-PIDΔSN (lanes 2 and 5), or pAS2-1-PIDΔAN (lanes 3 and 6). A, Two in-frame deletions of PID were used, one that deletes amino acids 69 to 82 and another that deletes residues 82 to 283 (lane 6). B, α-Galactosidase assays using the above-mentioned combinations.
