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In vitro interaction of PBP1 and TCH3 with PID. A, Western-blot analysis with anti-His antibodies detects His-tagged PBP1 and TCH3 after pull down with GST:PID from the soluble fraction of Escherichia coli extracts. GST alone is used as a control for the specificity of binding. B, Loading differences visualized by Coomassie Blue staining of GST:PID and GST. The His-tagged-interacting proteins are not sufficiently abundant to be visualized by this staining method.
